Journal: Bioactive Materials

Article Title: Bio-printing of aligned GelMa-based cell-laden structure for muscle tissue regeneration

doi: 10.1016/j.bioactmat.2021.06.031

Figure Lengend Snippet: ( A ) Optical cross-sectional images, ( B ) circularity values, and ( C ) percentage degradation of structures fabricated under different UV doses (75, 368, 821, 2106 mJ/cm 2 ) with a fixed UV exposure (config-2). ( D ) Immuno/fluorescent images of live (green)/dead (red), DAPI (blue)/phalloidin (red), and DAPI (blue)/MHC (green) images at day 3, 7, and 14, respectively. ( E ) MTT assays of C2C12 proliferation at 1, 3, and 7 days. Measured ( F ) cell viability at day 1, ( G ) cell density and ( H ) aspect ratio at day 7, and ( I ) positive MHC index at day 14. (n = 6, *p < 0.05, **p < 0.005, and ***p < 0.001). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: The solution was mixed with the C2C12 murine skeletal muscle cell line (CRL-1772; ATCC, Manassas, USA) or Adipose-derived stem cells (PT-5006; Lonza, Basel, Switzerland) at a density of 1 × 10 7 /mL.

Techniques:

Journal: Nature Communications

Article Title: Semi-solid prodrug nanoparticles for long-acting delivery of water-soluble antiretroviral drugs within combination HIV therapies

doi: 10.1038/s41467-019-09354-z

Figure Lengend Snippet: In vitro metabolism of emtrictabin (FTC) prodrugs under physiologically relevant conditions. a Initial rates of esterase-mediated hydrolysis of 5’-carbonate (1 mM, 1 – 8 ) to generate corresponding carbamates, and initial rates of hydrolysis of carbamates (1 mM, 9 – 16 ), resembling capecitabine, to generate FTC was monitored in undiluted human plasma ( b ), muscle S9 ( c ), and liver S9 ( d ) fractions. The light blue band represents the mean ± SD for carbamate cleavage from capecitabine

Article Snippet: Reaction mixtures containing mixed gender human skeletal muscle S9 (10.47 mg mL −1 , Bioreclamation) or phosphate buffer (0.1 M, pH 7.4) and pooled, mixed gender liver S9 (2.0 mg mL −1 , Xenotech) were pre-incubated at 37 °C for 5 min.

Techniques: In Vitro, Clinical Proteomics